3′–5′ Proofreading-induced detection of point mutations by PCR usingTliDNA polymerase
نویسندگان
چکیده
منابع مشابه
Modified Proofreading PCR for Detection of Point Mutations, Insertions and Deletions Using a ddNTP-Blocked Primer
The development of simple, accurate, rapid and cost-effective technologies for mutation detection is crucial to the early diagnosis and prevention of numerous genetic diseases, pharmacogenetics, and drug resistance. Proofreading PCR (PR-PCR) was developed for mutation detection in 1998 but is rarely applied due to its low efficiency in allele discrimination. Here we developed a modified PR-PCR ...
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Separation of DNA strands facilitates detection of point mutations by PCR-SSCP.
Single-strand conformation polymorphism (SSCP) is a powerful technique used to identify mutations in short pieces of DNA (1). In this technique, the DNA of interest is often amplified by the polymerase chain reaction (PCR) and then denatured by heat or alkali treatment before electrophoresis on a non-denaturing polyacrylamide gel. Differences in mobility of either of the single strands, compare...
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متن کاملProofreading dynamics of a processive DNA polymerase.
Replicative DNA polymerases present an intrinsic proofreading activity during which the DNA primer chain is transferred between the polymerization and exonuclease sites of the protein. The dynamics of this primer transfer reaction during active polymerization remain poorly understood. Here we describe a single-molecule mechanical method to investigate the conformational dynamics of the intramol...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1995
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/23.2.311